Mitochondrial disease (MD) is a heterogeneous group of pathologies caused by deficits in the ability of cells to generate sufficient energy to function. MD are neuromuscular diseases that mostly affect children, leading to severe multi-organ failure and death. Current treatments are limited to palliative care and vitamins or other supplements without clear indications of their effectiveness. MitoCBD has identified Cannabidiol (CBD), a compound found in cannabis without psychoactive activity, as a potential novel treatment for MD. The aim of the project is to develop an innovative treatment for MD based on repurposing cannabidiol (CBD), a drug with known anti-inflammatory, neuroprotective, and anti-epileptic effects.